Methods in functional proteomics: two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients, in-gel digestion and identification of proteins by mass spectrometry.
Methods in functional proteomics: two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients, in-gel digestion and identification of proteins by mass spectrometry. Methods Mol Biol. 2004; 250:263-82.
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Alkylation
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Alkylation
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Buffers
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Buffers
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Cell Line
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Cell Line
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Electrophoresis, Gel, Two-Dimensional
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Electrophoresis, Gel, Two-Dimensional
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Humans
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Humans
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Hydrogen-Ion Concentration
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Hydrogen-Ion Concentration
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Image Processing, Computer-Assisted
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Image Processing, Computer-Assisted
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Isoelectric Focusing
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Isoelectric Focusing
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Mass Spectrometry
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Mass Spectrometry
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Oxidation-Reduction
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Oxidation-Reduction
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Peptide Mapping
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Peptide Mapping
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Proteins
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Proteins
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Proteomics
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Proteomics
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Staining and Labeling
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Staining and Labeling
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Trypsin
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Trypsin